1. 百螢 鈣紫綠素500 CytoCalcein 405nm激發參數
E x(nm) 420
E m(nm) 505
分 子 量 517.93
溶 劑 DMSO
存儲條件 在零下15度以下保存，避光防潮
保 質 期 24個月
激發波長(nm) 410
發射波長(nm) 500
2. 百螢 鈣紫綠素500 CytoCalcein 405nm激發簡介及作用
鈣紫綠素500 CytoCalcein 405nm激發 是美國AAT Bioquest生產的細胞活力測定 試劑,CytoCalcein Violet 500設計用于以與鈣黃綠素AM相同的方式標記活細胞。它具有在405 nm處的最大激發,與流式細胞儀的紫色激光線完全匹配,也可以用熒光顯微鏡的激發源很好地激發。進入活細胞后,弱熒光的CytoCalcein Violet 500水解為強熒光染料,其最大激發/發射波長為405 nm / 500 nm。與典型的FACS熒光團的出色光譜分離為多重實驗提供了更多選擇。CytoCalcein Violet 450,CytoCalcein Violet 500,CytoCalcein Blue 550和CytoCalcein Blue 600已開發用于流式細胞儀。CytoCalcein 染料顯示出與Calcein,AM類似的生物學特性。它們針對各種流式細胞儀的激發波長進行了優化,為活細胞的流式細胞術分析提供了其他顏色。CytoCalcein Violet 450和CytoCalcein Violet 500被405 nm的紫色激光充分激發,并分別在450 nm和500 nm發射熒光。
3. 適用儀器
流式細胞儀
激發：405nm激光
發射：525/40nm濾波片
推薦孔板：AmCyan通道
熒光顯微鏡
激發：DAPI
發射：DAPI
推薦孔板：黑色透明
熒光酶標儀
激發：405nm
發射：500nm
Cutoff：475nm
推薦孔板：黑色孔板
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